ON STAINING FLUIDS, AND STAINING. 79 



stained by them should be mounted being given 

 with each formula. 



HJIMATOXYLIN STAINING FLUIDS. 



1. Haematoxylin (Crystals), 1 gramme. 

 Alum powdered, 10 grammes. 

 Distilled Water, 100 c.c. 

 Alcohol, 5 c.c. 

 A small piece of Camphor. 



This is a splendid nuclear stain. 



It does not become active until from two to three 

 months after it is made. It improves by keeping, 

 and remains perfectly good for two or three years. 

 It must be filtered each time it is used, and may be 

 used over and over again. Its full strength is too 

 strong for use ; lor rapid staining, i.e., in from five 

 to ten minutes, add to it an equal volume of distilled 

 water. For slow staining, dilute it until its colour 

 becomes a light violet, and leave the section in the 

 stain thus diluted for six to twelve hours. Over- 

 stained sections can be decolourised to any desired 

 tint in distilled water 5 parts, acetic acid 1 part. 



After staining and, if necessary, decolourising 

 the sections, place them in tap water for twenty- 

 four hours, and they will be found of a beautiful 

 blue tint. Mount in xylol balsam. 



2. EHRLICH'S FOEMULA. 



(a) Haematoxylin, 1 to 2 grammes. 

 Absolute Alcohol, 100 c.c.m. 



(6) Glycerine, 100 c.c.m. 



Distilled Water, 100 c.c.m. 



Glacial Acetic Acid, 5 c.c.m. 



Alum Sulph. (powdered), to saturation. 



Mix the two solutions. 



