ON STAINING FLUIDS, AND STAINING. 81 



sections, of which it is desired to produce lantern 

 slides or micro -photographs, may be stained by this 

 process. Mount in xylol balsam. 



4. KLEINENBERG'S FORMULA. 



(a) Prepare a saturated solution of chloride of 

 calcium in alcohol (of 70 per cent.), add a small 

 quantity of powdered alum, filter, mix 1 part of this 

 with 8 parts of 70 per cent, alcohol. 



(&) Make a saturated solution of haematoxylin 

 crystals in absolute alcohol. When using this for 

 staining purposes add to a small quantity of 

 solution (a), in a watch glass or saucer, sufficient of 

 (b) to give it the desired tint. 



This is a very fine nuclear stain, and permanent, 

 but the stain itself will not keep very long ; all 

 sections or tissues to be stained in it must be 

 thoroughly soaked in distilled water so that no 

 trace of acid may remain in them. 



Slow staining in a very dilute solution gives the 

 best results. Mount in xylol balsam. 



5. MITCHELL'S FORMULA. 



Take of finely ground Logwood, gii. 

 "Alum Potash, 5ix. 

 Glycerine, fl. %iv. 

 Distilled Water, quant, sun . 



Moisten the logwood with water until it is quite 

 damp. Place it in a large funnel with filter paper 

 and then pass ordinary water through it untilj the 

 liquid runs away almost colourless. When all the 

 water has drained from it, place the logwood on a 

 large dish to dry. 



Dissolve the alum in 8 ounces of distilled water, 

 6 



