82 METHODS OP MICROSCOPICAL RESEARCH. 



place the logwood in a vessel of sufficient size and 

 pour the alum solution upon it, allow it to remain 

 for forty-eight hours, stir it occasionally, strain it 

 off; add the glycerine and filter. Add 5 per cent, 

 of absolute alcohol to it, and keep it in a thoroughly 

 well stoppered bottle. For rapid staining this fluid 

 may be used full strength, but better results will be 

 obtained from prolonged immersion, say for twelve 

 hours, in a weak solution. This stain will give a 

 delicate violet tint to the sections with beautiful 

 clearness and differentiation. Mount in xylol 

 balsam. 



Carmine Staining Fluids. 



1. AMMONIA CARMINE. (Dr. Heale's Formula.) 



Carmine, 10 grains. 



Liquor Ammonia for t., B.P., | drachm. 



Price's Glycerine, 2 ounces. 



Distilled Water, 2 ounces. 



Alcohol, | ounce. 



Dissolve the carmine in the ammonia by means of 

 heat. Boil for some seconds and allow it to cool. 

 Let the ammonia evaporate for an hour or more, 

 and when the odour is nearly gone, add the 

 glycerine, water and alcohol, and filter. When, 

 from keeping, the carmine is precipitated, add one 

 or two drops of ammonia. It may be used full 

 strength or diluted with alcohol, which will increase 

 its penetrative power. This is a fine, nuclear stain. 



Mount in xylol balsam. 



2. RANVIER'S FORMULA. 



Distilled Water, 100 parts. 

 Ammonia, 1 part. 

 Carmine, 1 part. 



