ON STAINING FLUIDS, AND STAINING. 83 



Rub up the carmine in a mortar with a small 

 quantity of the water, add the ammonia, and when 

 the carmine is dissolved, add the remainder of the 

 water. If there is an excess of ammonia, use heat 

 until the carmine begins to be precipitated. 



Mount in xylol balsam. 



3. HUXLEY AND MAETIN'S FORMULA. 



Carmine, 2 grammes. 



Liquor Ammoniae/or., B.P., 4 c.c. 



Distilled Water, 48 c.c. 



Dissolve the carmine in the ammonia and water. 

 Place it in a bottle without a stopper until all odour 

 from the ammonia has nearly passed away. Keep 

 it in a thoroughly good stoppered bottle. When 

 used it should be diluted with from fifteen to twenty 

 times its bulk of water. 



Mount in xylol balsam. 



4. BORAX CAEMINE FLUID. 



(Foster and Balfour's Formula.) 



Carmine, 3 grammes. 

 Borax, 4 grammes. 

 Distilled Water, 97 grammes. 



Mix, apply heat with constant stirring until the 

 liquid nearly boils, set it aside to cool and when 

 cold, add 



Alcohol (Eectif.), 70 c.c. 



Distilled Water, 30 c.c. 



Shake it up thoroughly, let it stand for thirty-six 

 to forty-eight hours, and filter it. 

 Mount in xylol balsam. 

 Specimens stained in these carmine fluids may be 



