ON STAINING FLUIDS, AND STAINING. 87 



Stain the specimens in this fluid much diluted 

 with spirit. The stained specimens may be kept, to 

 be mounted as required, in spirit to which a small 

 quantity of this fluid has been added. 



This is a most valuable stain for brain, spinal 

 cord, nerve centres, and the nervous system gener- 

 ally, also for bone and cartilage. 



Mount in xylol balsam. 



Anilin Dyes as Staining Fluids. 



These are valuable for many purposes, and have 

 many useful properties. They stain rapidly, with 

 great clearness and brilliancy, and are especially 

 useful as double and triple stains in conjunction 

 with haematoxylin or carmine. 



No more beautiful or effective staining process 

 has been discovered than that in which the speci- 

 mens, having been stained in hsematoxylin fluid 

 subjected to the action of " tap " water for twenty- 

 four hours, until they have acquired a beautiful 

 blue tint, are afterwards stained in a weak solution 

 of 2 parts f uchsin and 1 part yellow eosin. 



This process gives a clear and pure triple stain. 



The student will find by experiment many equally 

 useful and beautiful combinations of these dyes. 



It has always been affirmed that most of the 

 anilin colours are more or less, and indeed, more 

 rather than less, fugitive, but the author ventures to 

 affirm as the result of his long experience that if 

 the necessary precautions are taken there is no 

 reason whatever why specimens stained with anilin 

 colours should not remain as permanent as any 

 others, and he has in his collection preparations so 



