90 METHODS OF MICROSCOPICAL RESEARCH. 



fill stains either singly or in conjunction with other 

 staining fluids, and especially with hgematoxylin ; 

 whilst, as before stated, yellow eosin combined with 

 fuchsin gives, with hsematoxylin as the ground 

 stain, a precise and beautiful triple stain. 



Eosin has great staining and especially penetrat- 

 ing powers, and specimens may remain in a weak 

 alcoholic solution for an indefinite time without fear 

 of their becoming over-stained. No trace of acid 

 must remain in any medium in which eosin stainings 

 are mounted ; but preparations mounted in xylol 

 balsam are practically permanent. 



Epithelium is beautifully stained by eosin and 

 the blood of the frog or triton double stained with 

 hsematoxylin and eosin forms a superb preparation. 



Iodine Green. Add to distilled water, in a 

 staining saucer, sufficient of the saturated solution 

 in alcohol to give it a fairly deep tint. Place the 

 specimens in distilled water for a short time before 

 staining them. Iodine green stains very rapidly, 

 and in many cases instantaneously. Wash them in 

 water, dehydrate in absolute alcohol, clear in xylol 

 and phenol, and mount in xylol balsam. Stained 

 specimens may remain in a weak alcoholic solution 

 of this dye indefinitely and be mounted as required. 



Methyl Green. This is an intense nuclear 

 stain, and Dr. Curschmann states that it has "a 

 peculiar affinity for amyloid substance, colouring it 

 an intense violet; whilst surrounding tissues that 

 have not undergone degeneration are stained green 

 or bluish green." 



Methyl Violet is also a valuable stain for 

 tissues which have undergone amyloid degenera- 

 tion : the amyloid substance is stained red, and the 

 unaffected parts violet. 



