ON STAINING FLUIDS, AND STAINING. 91 



Orange and Rubin. These combined, in 

 about the proportion of 1 part of the former to 2 

 parts of the latter, give, in conjunction with haema- 

 toxylin as the ground stain, a beautiful triple 

 stain. 



Saffranin is a good nuclear stain, imparting a 

 beautiful rose tint to the tissues, and staining the 

 nuclei a very deep red. 



Spiller's Purple is also a good nuclear stain, 

 and is a very restful colour for the eyes. 



Vesuvin, in conjunction with anilin blue, is very 

 effective as a stain for bacilli and bacteria. 



Specimens stained with anilin dyes must not be 

 cleared in oil of cloves or cedarwood oil, but exclu- 

 sively in xylol and absolute phenol in exactly equal 

 proportions. This medium clears almost instantane- 

 ously and perfectly, having also a preservative 

 rather than a destructive effect upon the anilin 

 colours of which it increases the brilliancy ; whilst, 

 moreover, the xylol balsam, in which they should 

 be mounted, exercises a preservative effect upon 

 the preparations, and which it renders practically 

 permanent. 



(Haematoxylin crystals, and the anilin dyes, are 

 supplied of the finest quality by Dr. G. Griibler, of 

 Leipzig, and can be obtained from his agent, Mr. C. 

 Baker, 244, High Holborn, London, W.C.) 



The Staining of Bacilli and Bacteria. 



To stain bacillus tuberculosis : 

 FORMULA No. 1. 



1. 1^ Fuchsin, 1 gramme. 



(a) Absolute Alcohol, 10 c.c. 

 Carbolic Acid, 5 grammes. 



(b) Distilled Water, 100 c.c. 



