92 METHODS OF MICROSCOPICAL RESEARCH. 



Mix the two solutions. (This remains active for 

 about two months.) 



2. Anilin (or Methylene) Blue, 2 grammes. 

 Absolute Alcohol, 15 c.c. 

 Distilled Water, 85 c.c. 



(This will keep a long time.) 



Spread a thin layer of sputum on a thin glass 

 cover, dry, and fix it over a spirit lamp or Bun sen's 

 gas burner until it commences to give off vapour; 

 then float the cover, sputum downwards, upon the 

 surface of a small quantity of fluid (1) in a staining 

 saucer ; leave it for fifteen minutes. 



In the case of sections, immerse them in the fluid 

 for twenty minutes. 



Now decolourise in 1 part of sulphuric acid to 

 7 parts of distilled water, until all colour has dis- 

 appeared. Wash away all trace of the acid in 

 distilled water, when a slight tinge of colour will 

 become perceptible. Float the cover upon, or 

 immerse the sections for a few minutes in, solution 

 2. Wash in distilled water, place in absolute 

 alcohol for three or four minutes. Let the cover 

 become perfectly dry, and mount in xylol balsam. 

 In the case of sections dehydrate them in absolute 

 alcohol, clear them in xylol and phenol, and mount 

 them in xylol balsam. (The xylol in which the 

 balsam is dissolved will clear the sputum on the 

 cover.) 



2. DR. HENEAGE GIBBES' FORMULA. 



1^ Magenta Crystals, 2 grammes. 

 Pure Anilin, 3 grammes. 

 Absolute Alcohol, 20 c.c. 

 Distilled Water, 20 c.c. 



