94 METHODS OF MICROSCOPICAL RESEARCH. 



WEIGERT'S PROCESS for Schizomycetes. 

 Bacilli, Bacteria and Micrococci. 



5t A 2 per cent, solution of Gentian Violet in Distilled 

 Water, 12 c.c., and a Saturated Aqueous Solution 

 of Anilin oil (filtered) 100 c.c. 



Stain the sections in this. 



Make a Solution of Vesuvin, 1 gramme. 

 Eectified Alcohol, sp. gr. 830, 10 c.c. 

 Distilled Water, 100 c.c. 



Stain in this for fifteen minutes. 

 Clear in xylol and phenol. 

 Mount in xylol balsam. 



REV. GORDON THOMPSON'S PROCESS. 



The special feature of this valuable process con- 

 sists in fixing the bacilli, bacteria or micrococci, 

 after staining them with the ground colour, by 

 subjecting them to the action of a solution of gold 

 made by dissolving 15 grains of gold chloride in 4 

 ounces of distilled water. This solution is to be 

 poured upon the cover, on which the bacilli have 

 been spread and allowed to remain upon it for two 

 or three seconds and then poured off again, the 

 cover is then to be washed in distilled water. The 

 gold appears to form a tube round the bacteria and 

 so encased, they appear to be rendered absolutely 

 permanent ; preparations, in the author's possession 

 mounted by this process twelve years ago, remaining 

 as brilliant in colour as the day they were made. 



For the ground stains 



Methyl violet, fuchsin and acid green are used, 

 the covers being floated upon the solution, washed 

 in distilled water, then treated with the gold 

 solution, and again washed in distilled water. 



