100 METHODS OF MICROSCOPICAL EESEAECH. 



tions hardened balsam may be re-dissolved in 

 turpentine all these solutions of balsam, whether 

 in xylol, benzol or turpentine must be carefully 

 filtered for use, and should be kept in wide-mouthed 

 capped bottles. 



1. GLYCERINE JELLY. 



1^ Glycerine (Price's), 50 c.c. 



Distilled Water, 42 c.c. 

 1 Boracic Acid (Crystals), 2 grammes. 

 Gelatine (Gold label), 6 grammes. 

 1 new laid Egg. 



Soak the gelatine in water for at least twelve hours. 

 Pour off the water, and place it in a vessel with the 

 distilled water. Melt it in a water bath. When 

 melted, add the glycerine and acid. Remove the 

 vessel from the water bath, beat up the white (only) 

 of the egg, and thoroughly incorporate it with the 

 fluid at a temperature of from 30 to 35 C. (not more). 

 Replace the mixture in the water bath, and gently 

 boil it until it has assumed a flaky appearance. 

 Now filter it through the finest filtering paper, 

 which must be done either in a warm oven, or with 

 a hot filter. Keep it in a well stoppered stock 

 bottle, and place some, for use, in a wide mouthed 

 capped bottle. When used, the bottle should be 

 placed in a bath of water sufficiently heated, and 

 kept so, to thoroughly liquify the " Jelly." 



1 Carbolic Acid (Crystals), or Formic Acid may be substi- 

 tuted for this ingredient the former, indeed, is generally 

 used but Boracic Acid does not affect hsematoxylin stainings, 

 which are unquestionably sooner or later much injured by the 

 other acids. 



