ON MOUNTING. 143 



which must be filtered and kept in a stoppered 

 bottle. When required for use dilute a portion 

 with four or five additional equivalents of water 

 and filter afresh. 



4. Stain in borax carmine solution for one to two 

 hours. 



5. "Wash in acidulated water (1 drachm nitric acid 

 to 1 pint of water). 



6. Wash quickly in pure water. 



7. Place in alcohol for one hour. 



8. Place in green stain for one to three hours. 



Acid Green, 2 grains. 

 Distilled Water, 3 ounces. 

 Glycerine, 1 ounce. 



Mix the glycerine and water well together and 

 dissolve the green crystals in the mixture, filter and 

 keep in a stoppered bottle. 



9. Wash quickly in absolute alcohol. 



10. Clear in xylol and phenol. 



11. Mount in xylol balsam. 



Another Process for Vegetable Sections. 



1. Place the section in an alcoholic solution of 

 iodine green (3 grains to the 1 ounce of alcohol) for 

 one or two hours. 



2. Soak it in alcohol for ten minutes. 



3. Remove it to water for one minute. 



4. Immerse it for two hours in carmine fluid, 

 made as follows : 



Carmine, 15 grains. 

 Ammonia, 15 grains. 

 Water, 2 ounces. 



Dissolve the carmine in the ammonia by means of 

 gentle heat, add the water, and filter. 



