BACILLUS MALLEI. 279 



ceeded in demonstrating the bacilli microscopically in the 

 blood of a patient suffering from glanders, and Philipo- 

 wicz was able to set up the disease in healthy animals 

 by injection of the urine of a guinea-pig suffering from 

 glanders. 



For the cultivation of the bacilli fresh nodules are most Cultivation, 

 suitable; nevertheless, pure cultivations have been obtained 

 from abscesses. On slices of boiled potato kept at 35 

 C., they form within 2 or 3 days a brownish, slimy, but 

 not very thick layer. The cultivations can be best pre- 

 served by inoculating mashed boiled potatoes, kept in 

 Erlenmeyer's flasks, in the form of a layer on the 

 bottom 1 to 2 cm. in thickness ; in these circumstances 

 a chocolate-brown layer is formed on the surface, which, 

 if protected from drying, contains for months living 

 bacilli or spores. Potatoes are the best nutrient 

 medium, and next to them comes solidified blood serum. 

 On serum kept at 37 C. we see, after three days, small 

 transparent discrete drops, which scarcely differ in colour 

 from the surface of the serum, but which distinctly pro- 

 ject above it. According to Weichselbaum and Kitt the 

 glanders bacilli also grow at about 25 C., although much 

 more slowly. On nutrient agar they form drop-like, soft, 

 greyish- white colonies ; in liquid nutrient jelly a tenacious 

 whitish mass is developed. 



Under the microscope the cultivations show the same 

 bacilli which we find in the glanders nodules ; as the 

 result of differences in age and development there are 

 greater differences in the lengtl >f Uio bacilli ; as a rule 

 spore formation can be distinctly seen. 



If the cultivations were inoculated into house-mice, no Experiments 

 result followed ; rabbits were partially susceptible, but in on amr 

 some only local ulcers formed, anu these subsequently 

 healed up. On the other hand the inoculation was always 

 successful in the case of field-mice, guinea-pigs, horses, 

 asses, and also in one sheep. Field-mice die after 

 subcutaneous inoculation with small quantities of the 

 cultivation within 8 days, and show on post-mortem 

 examination numerous small greyish-yellow nodules 

 full of bacilli in the spleen and liver. In the case of 



