RHINOSCLEROMA. 291 



this method the sections are stained for 24 hours in 

 fuchsine, washed in water, then placed for a few seconds 

 in pure or diluted solution of chloride of iron, washed in 

 alcohol, and then transferred to oil of cloves. The 

 syphilis bacilli remain red or reddish-violet, the tissue 

 and the other bacteria with the exception of tubercle 

 bacilli remain unstained. Double staining can also be 

 employed, both after this method and after Lustgarten's 

 plan, but it does not add much to the distinctness of the 

 picture. 



Doutrelepont and Schiitz were able to demonstrate 

 Lustgarten's bacilli by placing thin sections for 24 to 

 48 hours in a 1 per cent, watery solution of gentian 

 violet, then for a few seconds in dilute nitric acid (1 to 

 35), and then for about ten minutes in 69 per cent, 

 alcohol ; the tissues were afterwards stained in a dilute 

 watery solution of safranin. After this treatment the 

 tissues and nuclei had a bright-red appearance, while the 

 syphilis bacilli were blue. 



An investigation which has been recently made for Similar bacilli 

 purposes of control by Alvarez and Tavel in Cornil's mt 

 laboratory, throws doubt on the exclusive applicability of 

 Lustgarten's method of staining to the syphilis bacilli. 

 These authors found bacilli in the smegma of the prepuce 

 and of the vulva, which presented the same characters, 

 as regards staining, reaction, and morphological appear- 

 ance, as Lustgarten's bacilli. Hence further investi- 

 gations are required to demonstrate the causal connection 

 of these bacilli with syphilis, although their distribution 

 in the tissues is decidedly against the idea that they are-? 

 only accidental. 



RldnosclerGina. 



In this disease, which has been observed in Austria, Bacilli of 

 Italy, and Central America, and which is characterised 

 by thickening of the skin and mucous membrane of the 

 region of the nose and the formation of nodules, charac- 

 teristic micro-organisms were first described by Frisch, 



