820 



BACILLI PATHOGENIC IN ANIMALS. 



Inoculation 

 from animal 

 to animal. 



.Failure of 

 cultivation 

 experiments. 



Second 

 species. 



or three members. The blood containing the bacilli 

 when inoculated on mice in the quantity of about one 

 drop always produced the same disease in 80 inoculations 

 continued in series : on the first day no alteration could 

 be observed in the animals inoculated ; on the second 

 day they were lazy, they sat quietly with erected back, 

 and the eyelids were stuck together ; death occurred as 

 a rule after two or three days, at times, however, not 

 till after five days. On post-mortem examination nothing 

 abnormal was found, except very marked enlargement 

 of the spleen, and slight enlargement of the liver. In 

 sections of the organs small groups of bacteria were 

 found within the capillaries, but they were not particu- 

 larly numerous ; they were only present in very large 

 numbers in the lungs. Eabbits are relatively but little 

 susceptible; after inoculation with small quantities of 

 blood only a transitory illness resulted ; after subcuta- 

 neous injection of larger quantities of blood the animals 

 died after two or three days, and the bacilli showed the 

 same distribution as in the case of mice. Field mice 

 were very susceptible ; inoculation on a fowl was without 

 result. Cultivations were frequently attempted with the 

 most varied solid and fluid substrata, and at various 

 temperatures ; nevertheless they all failed, while control 

 experiments with the bacteria of rabbit septicaemia were, 

 without exception, successful. 



The second species was isolated in a similar manner 

 by the animal body (mice) from a deposit on the human 

 tongue. These organisms consisted of short rods rounded 

 at the ends, and slightly constricted in the middle ; 

 when stained they resemble a figure of 8, the stain being 

 more intense at the poles ; they are also surrounded by 

 a clear area, and on the whole they most closely resemble 

 the bacilli of chicken cholera. The rods were present in 

 large numbers in the blood of the heart of mice ; in sections 

 of the organs they were found inside the capillaries, but as 

 a rule in isolated masses, and not more numerous in 

 the lungs than in the other organs. The inoculation of 

 the disease on mice was carried on successfully through 

 40 or 50 generations with minute quantities of blood ; 



