BACILLUS CKASSUS SPUTIGENUS. 



323 



these points soon rising above the surface and then 

 spreading out in the form of greyish-white round gela- 

 tinous drops, which project considerably above the 

 surface of the jelly. Under a low power the youngest 

 colonies appear circular, of a greyish-brown dark colour 

 with dark points, or with short dark lines over the 

 whole surface. The superficial larger colonies appear 

 distinctly lighter, irregular in outline, and the surface. 

 more especially at the border is distinctly granular. 

 In puncture cultivations the organisms grow very 

 quickly, in about 24 hours, and show the typical nail- 

 shaped appearance. On potatoes they form a thick, 





Fig. 91. Bacillus crassus sputigenus (Kreibohm) X 700. 

 Cover glass preparation from the blood of the heart of a mouse. 



greyish-white moist and shining layer, similar to the 

 cultivation of the pneumonia bacilli, but somewhat 

 flatter and tougher, though as a rule scarcely distinguish- 

 able from the latter. Mice die after inoculation with Experiment 

 small quantities of the cultivation in about 48 hours ; on animals - 

 numerous bacilli are found in the blood of the heart, 

 and in all the capillaries, in largest numbers in the 

 liver. Babbits are not markedly affected by small doses, 

 if introduced by inoculation, but after intra -venous 

 injection of small doses they die within 48 hours, large 

 masses of bacilli being found in the blood. Large Action of 

 quantities of the cultivation injected into the veins larger doses- 

 cause in rabbits, and to a still more marked degree in 



