332 



BACILLI PATHOGENIC IN ANIMALS. 



be mentioned that very many of the other kinds of 

 bacteria, even the pathogenic kinds, when introduced 

 even in very large quantities into the blood stream, do 

 not produce this effect ; thus the typhoid bacilli, 

 bacillus tetragenus, the pyogenic cocci, the anthrax 

 bacilli, and many others when introduced in enormous 

 doses are either without, effect, or set up specific 

 diseases after they have gradually spread and multiplied 

 in the body. 



The following are some of the bacteria which set 

 up these intestinal symptoms in the most marked 

 manner : 



Toxic lactic 

 acid bacilli. 



Experiments 

 on animals. 



Bacillus oxytocus perniciosus (Wyssokowitsch) . 



These organisms were isolated by Wyssokowitsch in 

 the author's laboratory from milk, which had stood 

 there for a considerable time. They present the form 

 of short bacilli with rounded ends, somewhat thicker 

 and shorter than the ordinary lactic acid bacteria. They 

 form on gelatine plates at the deeper parts small 

 yellow colonies, which under a low power have a circular 

 appearance, are sharply outlined, finely granular, and 

 brownish -yellow in colour. On the surface the colonies 

 spread to the extent of about 1-J mm., they are greyish- 

 white in colour, round, raised, and show under a low 

 power a sharp outline, and light brown colour. In 

 puncture cultivations a nail-shaped growth is formed at 

 first, at a later period the cultivation extends super- 

 ficially over the whole surface of the gelatine. In stroke 

 cultivations a rapidly increasing deposit is formed of a 

 yellowish -white, or greenish colour. A small quantity 

 of the cultivation added to sterilised milk causes co- 

 agulation and acid reaction within 24 hours. Neither 

 the milk nor the cultivations have any odour. Small 

 doses inoculated on mice or rabbits have no effect at 

 all. Mixtures prepared from one or two test tubes, and 

 injected into the aural vein of rabbits, caused in each of 

 six cases death within three to twenty-two hours. Violent 

 diarrhoea set in about three-quarters of an hour after 



