336 



BACILLI PATHOGENIC IN ANIMALS. 



Cultivations. 



Microscopical and at times forming egg-shaped or even more elongated 

 cells, without or with very slight spontaneous move- 

 ment. In certain nutrient solutions (cane sugar, extract 

 of meat, and peptone) longer rods and threads are formed, 

 according to Buchner ; in glycerine jelly various in- 

 volution forms appear, characterised by slight thicken- 

 ing of the rod, and by staining only at the ends. On 

 gelatine plates the bacilli form colonies in the substance 

 of the jelly, which present the appearance, under a low 

 power, of round, and later irregular, almost egg-shaped 

 colonies with sharp outlines, of a brownish-yellow colour, 

 highly refracting, and distinctly granular in the interior. 

 On the surface of the gelatine the colonies spread to 

 about ten times the diameter of the deeper ones, and lie 

 on the surface like scales, of a cartilaginous or ground 



glass appearance, showing 

 rainbow colours with trans- 

 mitted light. Under a 

 low power it can be seen 

 that the limiting line is 

 markedly irregular or lo- 

 bular; the interior shows 

 two zones, a peripheral 



Fig 92. 



a. Preparation from a piece of mucus , , -, 



from the rice water contents of n 6, C 1 C a 1% C 1 U r 1 e S S, 



the human intestine x 700 faintly granular: and a 



b. Preparation from the peritoneal > h ' 



exudation of a guinea-pig which central One, yellowisll- 

 had died after subcutaneous in- 

 jection of the Naples bacteria 

 (stained with aniline fuchsine) X 

 700. (Both after Emmerich.) 



Experiments 

 on animals. 



brown towards the middle, 

 and equally granular. 

 Where the colonies are 

 more markedly developed, we can also notice radially ar- 

 ranged, though but slightly marked furrows (Buchner*). 

 On potatoes the bacilli grow at 37 C., in the form of 

 a brownish-yellow gelatinous layer. 



When Emmerich injected large quantities of the pure 

 cultivations of this organism under the skin, into the 

 lungs, or into the peritoneal cavity of guinea-pigs, death 

 occurred in from 30 to 42 hours, and, on examination, 

 hyperaemia of the intestinal and gastric mucous mem- 

 brane, and even hsemorrhagic erosions and superficial 

 * Arch. f. Hygiene, vol. iii., 3 Heft. 



