LABORATORY WORK IN DAIRY BACTERIOLOGY 265 



Bunsen turners. 



Forceps Common and Cornet forceps. 



Microscope, including a Vis-inch immersion lens and plenty of 



slides and cover glasses. 

 A counting plate or counting cards. 



MATERIALS 



Peptone. Phenolthalein. 



Salt. Alcohol. 



Beef extract. Corrosive sublimate. 



Gelatin gold label. NaOH. 



Agar Agar. HC1. 



Litmus, dry in cubes. Immersion oil. 



Absorbent cotton. Methylen blue. 



Common cotton, good quality. 



^ These solutions will best be bought from 

 Normal NaOH dealers. They may be made by methods 



[v described in No. 2 and 3, but the beginner 

 Normal HC1 | will hardly be able to make the Normal HC1 



J successfully. 

 Dextrose, lactose and saccharose. 



PRACTICAL WORK* 



*No. 1. Washing Glassware. All glassware used in bacte- 

 riological work must be thoroughly washed. No special direc- 

 tions need be given save that hot water and soap are necessary. 

 New glassware should be treated first with i% HC1. Used 

 glassware that contains the remains of gelatin or other media 

 must first be boiled in water, preferably containing a little sal 



1 The following laboratory manuals may be found useful as books of ref- 

 erence: 



Moore. Laboratory Directions for Beginners in Bacteriology. Ginn & Co., 

 1900. 



Frost. A Laboratory Guide in Elementary Bacteriology. The Macmillan 

 Co., 1903. 



Gorham. A Laboratory Course in Bacteriology. W. B. Saunders, 1901. 



Van Slyke. Methods of Testing Milk and Its Products. Orange judd Pub. 

 Co.. 1906. 



