LAHORATORY WORK IN DAIRY BACTERIOLOGY 293 



10 c.c in each. Sterilize by steaming one-half hour for three 

 successive days. 



No. 29. Testing Characters of Bacteria. Inoculate the sev- 

 eral bacteria previously isolated and purified, into various media. 

 In doing this it is best to make first a fresh agar slant from each 



FIG. 83 CUTTER FOR MAKING POTATO PLUGS, METHOD OF CUTTING 

 THEM AND PLACING THEM IN TUBES 



bacterium to be tested, so that the inoculation may be made 

 from a culture not over 24 hours old. Inoculate with a small 

 amount of the growth from the agar surface, the following: 



a. An agar slant, b. A gelatin stab. c. A tube of plain 

 bouillon, d. A dextrose fermentation tube. e. A lactose fer- 



