LIVING MATTER IN "PROTOPLASM." 25 



exposed to daylight, the yellow, shining substance which produces 

 the reticuluin in the protoplasm and its nucleus assumed a 

 violet color, while the substance within the meshes remained 

 uncolored. * 



Blood of the Newt (Triton). In a drop of blood of the newt, 

 transferred to the glass slide and covered with a covering-glass 

 oiled on its edges, we can observe the motion of the colorless 

 blood-corpuscles for hours at the temperature of the room. The 

 changes of shape result from a protrusion of light flaps and 

 granular offshoots of varying breadth, and sometimes of con- 

 siderable length, from the periphery of the protoplasmic body. 



Many finely granular bodies exhibit in their interior a con- 

 tinuous change of the grouping of the granules. Especially 

 after addition of a one-half per cent, solution of chloride of 

 sodium, during the locomotions of the body, vacuoles arise, and 

 the inner surface of the wall of many vacuoles looks jagged, as 

 if beset with torn points. For moments the whole body is 

 vacuoled, the smallest, just perceptible, vacuoles being transitions 

 to still smaller meshes, the filaments of which show pale gray 

 granules as points of intersection ; this appearance is only tem- 

 porary, as the next moment most, or even all, of the vacuoles 

 may have disappeared. 



In fresh blood, some coarsely granular colorless blood-cor- 

 puscles distinctly show filaments emanating from the granules. 

 With the assistance of an immersion-lens, No. 15 of Hartnack, 

 I became convinced that, during the locomotion of the corpuscle, 

 the single granules continually keep changing their size and 

 shape, as well as their location. 



In the blood of newts which had been kept all winter, the 

 nuclei of many colored blood-corpuscles were visible, both imme- 

 diately after the mounting of the specimen and after it had 

 remained a time on the glass slide. Each nucleus exhibits a 

 number of coarse, very shining granules, some of which show 

 filaments that are united with the neighboring granules. The 

 nucleus is bordered by a continuous layer of a substance of the 

 same refraction and color as the granules. There is often seen 

 around the nucleus a very narrow light rim, which at times 



* According to N. Lieberkiihn (Ueber Bewegungserscheinungen der 

 Zellen, 1870), in blood-corpuscles of many caterpillars there exists a space 

 between the contractile layer and the nucleus which is traversed by fila- 

 ments extending from the inner surface of the contractile layer to the outer 

 surface of the nucleus. 



