MODERN METHODS OF EXAMINATION. 69 



3. STAINING FLUIDS. 



Thiersch's Carmine Fluids. 



a. RED FLUID. 



1. Carmine, . . . . . . 1 part. 



Caustic ammonia, . *> "... '.,'-... 1 " 



Distilled water, . . . . . 3 parts. Filter. 



2. Oxalic acid, . . . . . . 1 part. 

 Distilled water, . * -v . . 22 parts. 



1 part of carmine solution to 8 parts of the acid solution, add 12 parts 

 absolute alcohol. Filter. After staining wash in 80 per cent, alcohol. 



b. LILAC FLUID. 



Borax, , ...-.. 4 parts. 



Distilled water, . . . . * . . 56 " 

 Dissolve and add, 



Carmine, . . . . . '.-.' 1 part. 

 Mix with twice the volume of absolute alcohol and filter. 



Beetle's Carmine Fluids. 



Carmine, . . . . . .10 grains. 



Strong liquor ammonia, . . ' . . \ drachm. 

 Glycerin, .... . . * " . 2 ounces. 



Distilled water, . . ' -.' v . 2 " ' i 

 Alcohol, . . . .. - , . \ ounce. 



Dissolve the carmine in the ammonia in a test-tube by aid of heat ; boil 

 it and cool and add the other ingredients. Filter. 



Acid Carmine Fluid. Mix ammoniacal solution of car- 

 mine with acetic acid in excess and filter. This is said 

 to stain diffusely, but adding glycerin with muriatic acid 

 (1 : 200), concentrates the color in the cell-nucleus. 



Anilin (or Magenta) Red Fluid. 



Fuchsin (crystal), 1 centigramme. 



Absolute alcohol, 20-25 drops. 



Distilled water, ..... 15 cubic centim. 



Anilin Blue Fluid. Anilin blue, treated with sulphuric 

 acid and dissolved in water till a deep cobalt color is 

 obtained. 



