Staining Animal Sections 151 



Ehrlich's Triple Stain for Blood Corpuscles. 



Saturated watery solution, orange * G ' ... 135 parts. 



methyl green 110 

 ,, ,, ,, acid fuchsin 100 ,, 



To the above add 



Glycerine ... ... ... ... ... 100 parts 



Absolute alcohol ... 200 



Distilled water 300 



This solution should stand for several weeks to allow for 

 sedimentation, and it improves with age. When used the 

 supernatant liquid should be drawn off with a pipette to 

 avoid the sediment. 



The cover-glasses are to be well cleaned with alcohol, and 

 the surface of one is touched with a drop of fresh blood, 

 and another cover-glass pressed on its surface until the 

 blood is evenly distributed. The covers are then separated 

 and allowed to dry. When dry they must be still further 

 hardened over a spirit lamp, or on a hot stage made of 

 sheet copper, and kept at 212 F. for from fifteen minutes 

 to two hours ; after which place in stain for from one to 

 four minutes, wash in water, dry, and mount in Canada 

 balsam, benzole, or xylol. 



The eosinophile granules in the corpuscles will be a 

 reddish hue, the neutrophile granules purple, and the 

 nuclei bluish green or blue. 



Toison's Solution for staining White Blood Corpuscles. 



Methyl violet ... ... ... J grain. 



Neutral glycerine ... ... ... 1 ounce. 



Distilled water 2 ounces. 



Mix thoroughly and add 



Chloride of sodium ... ... 15 grains. 



Sulphate of sodium ... ... 2 drams. 



Distilled of water 5J ounces. 



