Staining and Mounting Micro- Organisms 165 



together with twine and place in a jar of methylated spirit, 

 and soak for at least twenty-four hours. Then remove the 

 twine, separate the slides carefully, and place the specimen 

 in absolute alcohol for ten minutes to dehydrate. Clear in 

 clove-oil for one hour, and mount in Canada balsam. 



Anchylostoma. Harden in methylated spirit for ten 

 days ; then stain in borax carmine, wash in methylated 

 spirit, and place in acidulated alcohol to remove excess of 

 colour. Transfer to water, and soak until all trace of spirit 

 is removed ; then mount in glycerine jelly. 



Trichina Spiralis. Harden muscle with trichina 

 encysted in methylated spirit. Then embed in celloidin, 

 make longitudinal sections, stain in borax carmine, pass 

 through acidulated spirit ; then wash in water, and mount 

 in glycerine jelly. 



These worms may also be isolated from the muscle. 

 Tear out a piece of muscle on a glass slide with the aid of 

 a dissecting microscope or pocket lens, separate the capsule 

 containing the trichina from the muscle with a needle, and 

 place it in dilute hydrochloric acid until the capsule is 

 dissolved and the worm is set free ; then pick it up with a 

 fine sable brush, wash in water, and mount in glycerine 

 jelly. 



LESSON VI. 

 INJECTION OF BLOODVESSELS. 



Carmine and Gelatine Injecting Mass. 



Pure carmine ... ... ... 60 grains. 



Liq. ammonia fort. .. ... 2 drams. 



Glacial acetic acid ... ... 86 minims. 



Gelatine solution (1 ounce in 6 ounces 



of water) ... ... ... 2 ounces. 



Water ... ... ... ... 2 ounces. 



Dissolve the carmine in the ammonia and water in a test- 

 tube, and mix it with one-half of the warm gelatine. Add 



