R. H. A. PLIMMER AND W. M. BAYLISS. 



Firstly, autodigestion experiments of the trypsin, similar to those 

 above described, were carried out with the following results: 



(1) 2 grs. trypsin dissolved in water. 

 Filtrate 105 c.c. allowed to digest. 



Soluble P2^5 in ingm. 

 i9'9 

 21-6 

 23-1 

 20-7 

 19-8 



Total 2(5-0 



There is, thus, a considerable residue containing phosphorus which 

 does not pass into the form of soluble P 2 O 5 , even after several days' 

 digestion, so that the results of Sebelien and Biffi are confirmed. It 

 will also be observed that the soluble P 2 O 5 is already contained in the 

 trypsin solution as such, since on digestion this quantity is scarcely 

 increased. 



The residue, however, in the trypsin-caseinogen digest is not 

 altogether derived from the trypsin, since experiments on the digestion 

 by trypsin of paranuclein (the insoluble residue remaining when 

 caseinogen is digested by pepsin) showed that its phosphorus, which 

 amounted to 5'5 per cent. P 2 O 5 , was not completely converted into a 

 soluble form. Two experiments were made : 



