454 



R. H. A. P LI MM EH AND W. M. BAYLISS. 



50 grs. caseinogen suspended in 500 c.c. water + 500 c.c. HC1. Sp. gr. 1-16. 



Time 

 

 21 hours 



1 day 



2 days 



Soluble l'2<-> 5 in mgm, 



6-8 

 17-3 

 19-2 

 23-2 



Time 

 3 days 

 5 

 10 

 28 , 



(Soluble 1'aOs in iiigui. 



25-1 

 27-8 

 32-7 

 44-0 



Total 44-7 



We have not made any further experiments upon the action of acids 

 on caseinogen, since this subject has been investigated in great detail 

 by Goldschmidt (19) from another point of view, and we found that the 

 results obtained by the action of 1 per cent, alkali were of far greater 

 interest. 



Having found that 1 per cent, sulphuric acid produced no separa- 

 tion of soluble P 2 6 from caseinogen in 4 days, we investigated the 

 action of alkali of the same strength. A great difference was observed. 

 Soluble P0 5 was separated at a rate almost identical with that obtained 

 in the trypsiu digestions. The whole of the phosphorus was split off 

 in 24 hours, as the following data show : 



In every case the caseinogen was previously dissolved in a small 

 quantity of alkali. It might be supposed that even this small quantity 

 would cause a separation of soluble P 2 O 8 , but two experiments to deter- 

 mine this showed that no splitting-off of phosphorus occurred. In 

 24 hours in one experiment there was increase of 0'6 mgm. soluble P 2 O 5 , 



another experiment there was no increase of soluble P 2 O 5 after 



in 



21 hours. Thus, very dilute alkali sufficient only to dissolve the 

 caseinogen caused no splitting-off of soluble P 2 O 5 . The results show 



