DETECTION OF PHOSPHORUS. 123 



Table II shows that hydrochloric acid liberates the phosphorus in 

 a similar manner to nitric acid. The amount of nitrogen in the nitrate 

 was also determined. 



TABLE II. Ox testis, fresh. 880 c.c. HC1 (contained 130 c.c. HC1, 

 sp. gr. 1-16) added to 340 grs. of testis. Kept at 38. 



Inorganic phosphate at time of sample 5 = 18-3 mgms. P S 6 . 



Experiments were also made with other strengths of nitric and 

 hydrochloric acid and with sulphuric acid, but as the results show the 

 same general features the figures are not given. 



Much more interesting from our present standpoint were the 

 experiments carried out on testis which had been coagulated with 

 alcohol and then extracted with hot alcohol and ether until all the 

 phosphorus compounds soluble in these fluids had been removed. This 

 material still contained considerable quantities of inorganic phosphate, 

 but experiments with it showed that while, under the influence of acids, 

 the ' soluble ' phosphorus increased in the usual manner, the inorganic 

 phosphate remained constant. As these experiments were complicated 

 by this inorganic phosphate, which can readily be removed by extraction 

 with water, I will proceed to the experiments with the tissue from 



TABLE III. Ox testis, free from lecithin-like compounds, and also from 

 inorganic phosphates, i.e. testis containing presumably only nuclein 

 compounds of phosphorus. To 100 grs. of moist material 500 c.c. 



2N 



T 



~ HN0 3 added. 



Mgms. PjOs in 



50 c.c. of digest as Inorganic 



' soluble ' phosphorus phosphate 



Sample taken at commencement contained 2 '8 Absent 



after 24 hours contained 14-2 ,, 



i> 48 16-2 



,, 168 17-5 



Total phosphorus in 50 c.c. =24'0 



