TRYPSINOGEN AND ENTEROKINASE. 477 



The following mixtures were then made in small flasks : 



mm. of gelatine 

 digested 



^ 

 (1 



A. 2 c.c. Dog's P.J. + l c.c. Dog's Ek. 



+1 c.c. Cat's Ek. 



,, +1 c.c. Bat's Ek. 



,, + 1 c.c. Babbit's Ek. 



+1 c.c. Frog's Ek. 



,, +1 c.c. Pigeon's Ek. =14 



,, +1 c.c. Tortoise's Ek. = 13 



,, + 1 c.c. Dace's Ek. =14 



,, +0c.c. Ek. = 



B. 2 c.c. Babbit's P.J. + l c.c. Dog's Ek. =13 6 



,, +1 c.c. Cat's Ek. =13 6 



+ 1 c.c. Bat's Ek. =11 5-5 



+ 1 c.c. Babbit's Ek. = 12 6 



,, + 1 c.c. Frog's Ek. = 7 5'5 



,, +0 c.c. Ek. =00 



C. 2 c.c. Cat's P. J. + 1 c.c. Dog's Ek. = 13 



+1 c.c. Cat's Ek. =12 



,, + 1 c.c. Bat's Ek. =13 



+1 c.c. Babbit's Ek. =13 



+1 c.c. Frog's Ek. =13 



,, + 1 c.c. Pigeon's Ek. =12-5 



+1 c.c. Tortoise's Ek. = 13 



,, + 1 c.c. Dace's Ek. =13 



+0c.c. Ek. = 



Gelatine tubes and a drop of toluol were added to each flask and the 

 whole left for 16 hours. At the end of this time the amount of gelatine 

 digested is shown in the first column opposite each mixture. New tubes 

 were then added to series A and B and were measured after the lapse 

 of 6 hours. The results are given in column 2. 



Delezenne (2) has already shown that pancreatic extracts obtained 

 from various animals can be activated by dog's enterokinase. From the 

 above experiments we see that not only is this the case, but that entero- 

 kinase from a variety of animals is capable of activating pancreatic 

 juice obtained from different sources. It will be noticed that in A and 

 B the amount of digestion at the end of 16 hours in the tubes activated 

 by frog's enterokinase is less than that in the other tubes. That this is 

 due merely to the small quantity of enterokinase in the solution and not 

 to any real difference in the enterokinase itself is proved by the fact 

 that on the addition of new tubes digestion proceeds equally in all the 



