488 J. M. HAMILL. 



We see that worm extract to which disodium hydrogen phosphate 

 has been added is just as powerful an inhibitor of tryptic digestion as 

 ordinary worm extract. This phosphate solution, however, when boiled, 

 completely loses its anti-action, whereas ordinary worm extract after 

 similar treatment retains its activity unimpaired. 



Diffusibility of the antibody. This was investigated in the following 

 way. 15 c.c. of worm extract were dia'ysed against running water for 

 four days, whilst 15 c.c. of the same extract were kept as a control in a 

 flask with a little toluol. The 15 c.c. of dialysed worm extract became 

 diluted to 43 c.c., therefore the 15 c.c. of control extract were also diluted 

 to 43 c.c. and the following mixtures were made : 



Dig. in mm. 



2c.c. T + 2c.c. W =3-5 

 2 c.c. T + 2 c.c. DW = 7'0 

 2 c.c. T + 2 c.c. H 2 = 7'0 



Control worm extract = W. Dialysed worm extract = DW. Gelatine tubes and toluol 

 were added. The tubes were measured after 16 hours. 



From the fact that the worm extract loses all its inhibitory action 

 after dialysis we can conclude that the antibody is not colloidal in 

 nature, but, on the contrary, possesses the power of diffusibility to a 

 marked degree. 



The action of alcohol on worm juice. For these experiments worms 

 were thoroughly ground up with sand, mixed with kieselguhr, and 

 subjected to a pressure of 300 kgs. per sq. cm. in a Buchner's press. A 

 clear yellowish juice, acid in reaction, was thus obtained. Two volumes 

 of absolute alcohol were added to the juice and the heavy precipitate 

 which formed was filtered off. The precipitate was washed six times by 

 shaking with a mixture of two parts of alcohol and one of water; the 

 filtered aqueous extract was used in the experiments below as AP lt 

 The alcoholic filtrate was evaporated nearly to dryness in vacuo at a 

 temperature of 40 C. to get rid of the alcohol. The residue redissolved 

 in water is spoken of below as AE^ 



The solution APj was now taken, some was kept neutral (APj neut.) ; 

 some of this neutral solution was boiled (BAP X neut.) ; some was acidified 

 with HC1, boiled for 3 minutes, allowed to cool and then carefully 

 neutralised with ammonia (BAP! acid); finally, some was made just 

 alkaline with ammonia and boiled for 3 minutes (BAPj alk.) The 

 following mixtures were then set : 



