ANTIBODY OF INTESTINAL WORMS. 489 



Dig. in mm. 



2 c.c. T + 2 c.c. APi =3-0 



2 c.c. T + 2 c.c. BAPj neut. =3*5 

 2 c.c. T + 2 c.c. BAPj acid =3'0 

 2 c.c. T + 2 c.c. BAPj alk. = 8'0 

 2 c.c. T + 2 c.c. H 2 =8-0 



Gelatine tubes and toluol were added, the tubes were measured after 16 hours. 



The solution AEj was also examined for inhibitory action in the same 

 way as AP } . 



The following mixtures were made : 



Dig. in mm. 

 after 16 hrs. 



2 c.c. T + 2 c.c. AEj =3-0 



2 c.c. T + 2 c.c. BAEj acid = 3*0 

 2 c.c. T + 2 c.c. BAEj alk. =7'0 

 2 c.c. T + 2 c.c. H 2 =7'0 



From these two experiments we see that both the precipitate and 

 filtrate, obtained by the addition of two volumes of absolute alcohol to 

 the worm juice, contain antibody. Prolonged washing of the precipitate 

 with alcohol of this strength does not remove the inhibitory substance. 

 The probable explanation of this is that some of the antibody is carried 

 down by the precipitate and held there by a process analogous to 

 adsorption, and in this state 66 /o alcohol is unable to remove it from 

 the precipitate. 



Since the alcoholic nitrate is acid in reaction, we can evaporate the 

 filtrate to dryness on the water-bath instead of boiling off the alcohol in 

 vacuo as described above. The aqueous extract obtained by treating 

 the residue with water is strongly inhibitory, as is seen from the follow- 

 ing experiment : 



Dig. in mm. 

 after 16 hrs. 



2 c.c. T + 2 c.c. AEj =3'0 



2 c.c. T + 2 c. c. BAEj acid = 3 -0 

 2 c.c. T + 2 c.c. BAEj alk. --=7 '7 

 2 c.c. T + 2 c.c. H 2 =7'7 



If we now take the alcoholic filtrate obtained by treating worm juice 

 with 2 volumes ot absolute alcohol (AEj) and add to it a further quantity 

 of alcohol (2 volumes) a very fine precipitate amounting to hardly more 

 than a turbidity is produced. Eventually, however, the scanty precipi- 

 tate settles to the bottom of the vessel, leaving the alcoholic solution 

 quite clear. No further precipitate is produced by adding more alcohol. 

 The precipitate was filtered off and washed with alcohol. It dissolved 

 readily in water, the solution so obtained being here called AP 2 . 



