490 J. M. HAM ILL. 



The alcoholic filtrate from this precipitate was evnporated almost to 

 dryness in vacuo at 40 C. The residue dissolved in water is called AE. 2 . 



The solution AP 2 was now taken ; some was made acid with HC1, 

 boiled for 3 minutes and then cooled and neutralised (BAP 2 acid); some 

 was made faintly alkaline with ammonia and boiled for 2 minutes 

 (BAP 2 alk.); and some was kept untouched (AP 2 ). 



The aqueous AE 2 was treated in the same way as AP 2 and the 

 following mixtures were made : 



Mm. digested 



2 c.c. T + 2c.c. AP 2 -2-5 



2 c.c. T + 2 c.c. BAP 2 acid = 2 -5 

 2 c.c. T + 2 c.c. BAP 2 alk. = 6-5 

 2 c.c. T + 2 c.c. H.,0 =7'0 



Mm. digested 



2 c.c. T + 2 c.c. AE 2 =6-0 



2 c.c. T + 2 c.c. BAE 2 acid = 6'0 

 2 c.c. T + 2 c.c. BAE 2 alk. = 7'0 

 2 c.c. T + 2 c.c. H.,0 =70 



Toluol and gelatine tubes were added and the digestion measured after 16 hours. 



We see from these experiments that the fraction AP 2 precipitated 

 by the addition of further alcohol is extremely active in inhibiting 

 tryptic digestion ; further, practically all the antibody is thrown down by 

 this concentration of alcohol, since the filtrate possesses only an insigni- 

 ficantly small inhibiting action. 



To the clear alcoholic filtrate AE 2 , three volumes of ether were added. 

 A scanty granular precipitate was thrown down. This was filtered off, 

 washed with ether, and dried. It dissolved easily in water, forming a 

 clear solution, EP. The ether filtrate was evaporated nearly to dryness 

 in vacuo, and the residue dissolved in water, EE. 



The following mixtures were then made : 



Dig. in mm. 

 after 16 hrs. 



2 c.c. T + 2 c.c. H 2 - 7-0 

 2 c.c. T + 2 c.c. EP = 7-0 

 2 c.c. T + 2 c.c. EE = 6'0 



Gelatine tubes and toluol were added and the digestion measured after 16 hours. 



The ether extract is thus practically without inhibitory power, the 

 ether precipitate is quite inactive. The solution AEj gives the biuret 

 reaction and Fehling's test. AE 2 gives Fehling's test but no biuret 

 reaction. The solution EE gives no Fehling's test or, at the most, gives 

 a very slight reduction. The precipitate EP however reduces Fehling's 

 solution very strongly. 



We are able, therefore, by means of the addition of alcohol, to 

 precipitate first of all most of the proteid from the worm juice. Both 

 this precipitate and the alcoholic filtrate obtained from it exert a 



