100 R. H. A. PLIMMER. 



EXP. I. 60 c.c. pancreatic juice. 



100 c.c. 5/ lactose solution + 25 c.c. juice + toluol. Control 100 c.c. 5/ lactose 



N 

 solution + 25 c.c. boiled juice + toluol. Incubated at 38 for 24 hours. 25 c.c. H 2 S0 4 



then added to each. 



50 c.c. removed and precipitated by 10 c.c. mercuric nitrate. Filtered; 50 c.c. filtrate 

 neutralised with 5 c.c. 10/ NaOH and filtered. 45 c.c. filtrate treated with H 2 S. Excess 

 of H 2 S removed with CuS0 4 . Made up to 100 c.c. Filtered ; and filtrate used for rotation, 

 reduction, and osazones after neutralising and filtering from Cu (OH) 2 . 



Remainder of neutralised mixtures incubated for 24 hours longer and then precipitated 

 by 10 c.c. mercuric nitrate solution. Filtered; 100 c.c. filtrate neutralised with 6 c.c. 

 10/ NaOH and filtered. 90 c.c. filtrate treated with H 2 S. Excess of H 2 S removed by 

 CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used for rotation, reduction, and 

 osazones after neutralising and filtering from Cu(OH) 2 . 



The osazones both of the experiment and the control were completely soluble in boiling 

 water. They melted at 210 212 C. and 209 210 C. respectively; 0-1954 grm. osazone 

 obtained from the experiment gave 16-4 c.c. N at 14 C. and 772mm. Hence N = 9 < 98/ . 



EXP. II. 125 c.c. toluol water extract of pancreas of milk- and lactose-fed dog. 

 JlOOc.c. 5 / lactose solution, 50 c.c. extract + 2 c.c. toluol ) Incubated at 38 C. 



}lOO c.c. 5/ lactose solution, 50 c.c. boiled extract + 2 c.c. toluol) for 48 hours. 



To each 10 c.c. mercuric nitrate solution added. Filtered; 140 c.c. filtrate neutralised 

 with 6-5 c.c. 10/ NaOH and filtered. 125 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used for rotation, 

 reduction, and osazones after neutralising and again filtering. 



The osazones from both solutions were completely soluble in boiling water and both 

 melted at 208210 C. 



0-2626 gm. osazone of the experiment gave 23 c.c. N at 14 C. and 770 mm. Hence 

 N = 10-44 / . 



0'2560gm. osazone of the control gave 21 '9 c.c. N at 13 C. and 762mm. Hence 

 N = 10-41 / . 



EXP. III. 120 c.c. toluol water extract of pancreas of kitten 4 6 weeks old. 

 J100 c.c. 5/ lactose solution, 50 c.c. extract, 2 c.c. toluol 1 Incubated at 38 C. 



}lOO c.c. 5 / lactose solution, 50 c.c. boiled extract, 2 c.c. toluol j for 53 hours. 



To each 10 c.c. mercuric nitrate solution added. Filtered ; 140 c.c. filtrate neutralised 

 with 6 c.c. 10 % NaOH and filtered. 120 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used for rotation, 

 reduction, and osazones after neutralising and filtering. 



The osazones in both cases were completely soluble in boiling water. 



EXP. IV. 120 c.c. chloroform water extract of intestine of kitten. 



JlOOc.c. 5/ lactose solution, 50 c.c. extract, 2 c.c. toluol 1 Incubated at 38C. 



(100 c.c. 5/ lactose solution, 50 c.c. boiled extract, 2 c.c. toluol) for 53 hours. 



To each added 10 c.c. mercuric nitrate solution. Filtered. 140 c.c. filtrate neutralised 

 with 6 c.c. 10 % NaOH and filtered. 120 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used for rotation, 

 reduction, and osazones after neutralising and again filtering. 



The osazone of the experiment was not completely soluble in boiling water. The 

 insoluble portion was recrystallised from dilute alcohol. It melted at 205 207 C. 



0-1020 gm. gave 13-9 c.c. N at 14 C. and 768mm. Hence N = 16-2 / . 



The osazone of the control was completely soluble in boiling water. 



