ADAPTATION OF PANCREAS 101 



EXP. V. 70c.c. pancreatic juice of milk- and lactose-fed dog. (2 c.c. =2-33 c.c. 

 0-4/ HCl). Neutralised with 77 c.c. 0-4 / HC1. 



JlOO c.c. 5/ lactose solution, 60 c.c. juice, 2 c.c. toluol 1 Incubated at 38 C. 



(100 c.c. 5/ lactose solution, 60 c.c. boiled juice, 2 c.c. toluol J for 76 hours. 



To each added 10 c.c. mercuric nitrate solution. Filtered. Filtrate not completely 

 precipitated, so that another 30 c.c. mercuric nitrate added to 160 c.c. nitrate. Filtered ; 

 180 c.c. nitrate neutralised with 24 c.c. 10 / NaOH and filtered. 190 c.c. filtrate treated 

 with H 2 S. Excess of H 2 S removed with CuS0 4 . Made up to 250 c.c. Filtered ; and filtrate 

 used for rotation, reduction, and osazones after neutralising and filtering. 



The osazones of the experiment and the control were completely soluble in boiling 

 water. They melted at 208 210 C. and 209 211 C. respectively. 



0-2628 gm. of osazone of the experiment gave 22 -3 c.c. N at 13 C. and 770mm. 

 Hence N = 10-17 / . 



EXP. VI. 150 c.c. toluol water extract of pancreas of milk- and lactose-fed dog. 



(100 c.c. 5 / lactose solution, 60 c.c. extract, 2 c.c. toluol 1 Incubated at 38 C. 



[100 c.c. 5/ lactose solution, 60 c.c. boiled extract, 2 c.c. toluol f for 76 hours. 



To each added 10 c.c. mercuric nitrate solution. Filtered; 140 c.c. filtrate neutralised 

 with 6 c.c. 10% NaOH and filtered. 125 c.c. filtrate treated with H 2 S. Excess? , of H ? S > 

 removed with CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used fcr' rottttiofr,' 

 reduction, and osazones after neutralising and again filtering. .' ' __ . 'i, 



The osazones in both cases were completely soluble in boiling water. , ." ' \ ; . , j j ' \ 



EXP. VII. 25 c.c. pancreatic juice of milk- and lactose-fed dog. 



JlOO c.c. 5 / lactose solution, 10 c.c. juice, 2 c.c. toluol 1 Incubated at 38 C. 



(100 c.c. 5/ lactose solution, 10 c.c. boiled juice, 2 c.c. toluol J for 4 days. 



To each added 10 c.c. mercuric nitrate solution. Filtered ; 100 c.c filtrate neutralised 

 with 5-5 c.c. 10 % NaOH and filtered. 90 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 250 c.c. Filtered; and filtrate used for rotation, 

 reduction, and osazones, after neutralising and again filtering. 



The osazones in both cases were completely soluble in boiling water. 



EXP. VIII. 175 c.c. toluol water extract of pancreas of milk- and lactose-fed dog. 

 (100 c.c. 5/ lactose solution, 75 c.c. extract, 2 c.c. toluol | Incubated at 38C. 



llOOc.c. 5/ lactose solution, 75 c.c. boiled extract, 2 c.c. toluol f for 80 hours. 



To each added 10 c.c. mercuric nitrate solution. Filtered. 150 c.c. filtrate neutralised 

 with 6 c.c. 10/ NaOH and filtered. 135 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 250 c.c. Filtered ; and filtrate used for rotation, 

 reduction, and osazones after neutralising and again filtering. 



The osazones in both cases were completely soluble in boiling water. 



EXP. IX. 70 c.c. toluol water extract of mucous membrane of intestine of dog. 

 (100 c.c. 5/ lactose solution, 25 c.c. extract, 2 c.c. toluol \ Incubated at 38C. 



jlOOc.c. 5/ lactose solution, 25 c.c. boiled extract, 2 c.c. toluol j for 64 hours. 



To each added 15 c.c. mercuric nitrate solution. Filtered. 120 c.c. filtrate neutralised 

 with 8-1 c.c. 10/ NaOH and filtered. 20 c.c. filtrate treated with H 2 S. Excess of H 2 S 

 removed with CuS0 4 . Made up to 100 c.c. Filtered; and filtrate used for rotation and 

 reduction. Remainder of solution treated in same way but used for osazones after 

 neutralising and again filtering. 



The osazone of the experiment was not completely soluble in water. The insoluble 

 portion was recrystallised from dilute alcohol. It melted at 195 198 C. 



0-1038 gm. gave 12 c.c. N at 11 C. and 768 mm. Hence N = 13-93 / . 



The osazone of the coutrol was completely soluble in boiling water. 



