LAGTASE IN INTESTINE. 



27 



Table of Results (continued). 



Control 



tine, filtered through 

 Berkefeld filter 



(53) Pig, fed 3 months with 



milk, lower half intes- 

 tine, filtered through 

 Berkefeld filter 



(54) Pig, fed 3 months with 



milk, pancreas 



(55) Pig, fed 3 months with 



milk, pancreas + solu- 

 tion of glucose instead 

 of lactose 



(56) Pig, pancreas ... 



(57) Pig, pancreas, solution of 



glucose instead of lactose 



0-2464 

 0-2464 



0-2352 

 0-2372 



0-3576 

 03582 



0-2770 

 0-2788 

 0-1874 

 0-1870 



103-3 



98- 



155-1 



117-9 



77-3 



0-2436 

 0-2430 



101-9 



0-2244 



0-2258 

 diff. = 4-9 mgm. 



0-3530 



0-3544 

 diff. = 2-2 mgm. 



0-2666 

 0-2684 



0-1858 

 0-1872 



93-9 



..a. 



112-8 



76-9 



3-2 



12-2 



10-6 



In all the experiments the period of incubation of the extracts of 

 intestine with lactose lasted from 2 4 days, a length of time which is 

 necessary for the animal lactase to exert its influence, especially when 

 only a small quantity is present and particularly when only about 

 20 30 per cent, inversion occurs. The following experiment, made to 

 determine the rate of action of lactase in the intestine of the cat, shows 

 clearly that this length of time of incubation is necessary. (Weinland 

 incubated his extracts only for a few about 6 hours.) 



150 c.c. 5 / lactose solution, 150 c.c. extract, 2 c.c. toluol. After certain intervals of 

 time 25 c.c. were removed and precipitated by 5 c.c. Hg(NO 3 ) 2 . ' Filtered. 20 c.c. filtrate 

 neutralised with 2-5 c.c. 10 / NaOH and filtered. 15 c.c. filtrate treated with H 2 S. 

 Excess of H 2 S removed with CuS0 4 . Made up to 50 c.c. and filtered. 20 c.c. for 

 reductions. 



