CHEMICAL DYNAMICS OF ANIMAL NUTRITION 141 



expected from the generalisations made below that changes other 

 than those affecting the nitrogen take place in the tissues ; the results 

 obtained show no parallelism between the physical and the nitrogenous 

 changes. 



Five grams of tissue were allowed to stand for a short time with 

 50 c.c. of cold water, made just acid with one drop of acetic acid. 

 A part of the extract was, in each case, heated rapidly to 100, in a 

 closed vessel (so that no evaporation took place), and maintained at 

 this temperature in a boiling water bath for 15 minutes. The 

 determinations were made with boiled and unboiled extracts. 



Liver. Electrolytic conductivity 



Unboiled extract R = 495*2, c = 651-670, k = 1317 

 Boiled extract R = 443*5, c = 651*670, k = 1469 



Difference ... 152 



Small Intestine. Electrolytic conductivity 



Unboiled extract R = 320-9, c = 651-670, k = 2031 

 Boiled extract R = 2906, c = 651-670, k = 2242 



Difference ' ... 211 



Freezing point 



Unboiled extract ... A = O'o8 

 Boiled extract ... A = 0-09 



Another experiment was made. Five Grams of tissue were boiled 

 for half hour with 50 c.c. alcohol. The latter was decanted off, and 

 the precipitate washed several times with hot water. The washings, 

 both alcoholic and aqueous, were then evaporated to a small bulk in 

 vacua, and dissolved in water. This solution was made up to 32 c.c. 

 The freezing point of this solution was then compared with the 

 freezing point of an extract of 5 grs. tissue in 30 c.c. cold water. 

 Result : 



A For tissue extract ... =0-17 

 A For extractives solution = 0-17 



