i 4 4 BIO-CHEMICAL JOURNAL 



PART V 



FURTHER RESEARCHES ON AUTOLYSIS : THE RELATION OF 

 AUTOLYSIS TO NUTRITION 



Section A. Auto lysis of the Liver 



The method employed in the following researches is essentially 

 that described in the first paper 1 (Lane-Clayppn and Schryver, loc. cit.~) 



One or two minor modifications were made. After precipitating 

 with trichloracetic acid, the mixture was allowed to cool. It was 

 filtered when cold, after standing for several hours ; the filtrate was 

 washed with hot water and, in the event of the bulk, not being too 

 great, directly evaporated down with sulphuric acid in the Kjehldahl 

 flask. 



Except when otherwise stated, the numbers given under the 



heading ' autolysis ' refer to the number of c.c.-jg- H 2 so* necessary for 

 neutralisation of NH ? obtained in the Kjehldahl analysis. Four grams 

 of tissue were used for each experiment. 



Series I. Effect of Serum. In all the experiments with serum, a 

 portion was incubated with saline as a control (same volume as liquid 

 in actual experiment). After incubation, serum in same dilution (also 

 incubated for the same time) was added to the control ; this mixture 

 was then rapidly heated to boiling, and trichloracetic acid in definite 

 quantity added. The same amount of saline as was used for the 

 control was added to the portion incubated with serum. The mixture 

 was then boiled, and the same amount of trichloracetic acid added a s 

 was used in the control. By this means coagulation in the actual 

 experiment and the control were carried out under absolutely identical 

 conditions. 



Sheep's Serum on Cafs Liver. 20 c.c. liquid to 4 grams liver. 



A star indicates total N in filtrat: from coagulum (includes residual N, N from products of tryptic 

 digestion, etc.) 



