146 fitO-CHEMICAL JOURNAL 



The control was made by incubating with saline, and then 

 adding the same quantity of products of tryptic digestion as was used in 

 the actual experiment. The portion incubated with the products of 

 digestion was diluted before coagulation with the same amount of 

 saline as was used for the control. The filtrate in which N was 

 estimated includes, of course, the products ot digestion as well as 

 those of autolysis. 



N 

 5 c.c. trypsine solution = 36*0 c.c. H 2 SO 4 (Kjehldahl) 



20 c.c. saline + 5 c.c. to 4 grains of liver ; control in 25 c.c. saline. 



Tirriw With products of digestion Control Difference 



7 hours 48 o 58*0 lO'O 



8 57-7 65-0 7-3 



9 6z '7 66-3 3-9 



Series 111 (^). To test the effect of the quantity of the products 

 of digestion on autolysis rate. The control in this case represents the 

 N of the filtrate obtained by boiling the tissue with the products of 

 tryptic digestion, adding trichloracetic acid, etc., without previous 

 autolysis. 



N 

 T.D. = products of tryptic digestion. (5 c.c. = 36 c.c. H 2 SO 4 ) 



S = Saline 



Solution used Time * N in filtrate Autolysii 



20 c.c. T.D. No saline. o hours (control) H7'5 



7 hours 1 53'2 57 



9 hours 1 5 7 - 5 10*0 



10 c.c. T.D. + 10 c.c. S. o hours (control) 77^2 



7 hours 87-0 9-8 



9 hours 89^4 I2'2 



5 c.c. T.D. + 15 c.c. S. o hours (control) 387 



7 hours 53'3 14*6 



9 hours 57-2 185 



5 c.c. T.D. + 35 c.c. S. o hours (control) 4"4 



7 hours 54-3 13-9 



9 hours 57-2 ' i6 - 8 



These figures indicate that the rate of autolysis is influenced by 

 the quantity of the products of tryptic digestion, but little by the 

 dilution. 



