viii Contents. 



TACK 



Eyepiece micrometer and stage micrometer Jackson's micro- 

 meter Rarnsden screw micrometer Botterill's trough 

 Rousselet's live-cage Stage forceps Text-books recommended 38-68 



P ART II. 

 LESSON 1. 



HARDENING ANIMAL TISSUES FOR MICROSCOPICAL EXAMINATION. 



Absolute alcohol Chromic acid and spirit Potassium bichromate 

 Ammonium bichromate Miiller's fluid Methylated spirit 

 Decalcifying fluid General directions for hardening - - 69-71 



LESSON 2. 



EMBEDDING TISSUES AND SECTION-CUTTING. 



Cutting sections with a razor by hand Embedding in paraffin, 

 wax and lard Infiltrating a tissue with paraffin Cole's micro- 

 tome and embedding in carrot Cathcart microtome for freezing 

 Embedding in celloidin Cambridge rocking microtome - 72-77 



LESSON 3. 



STAINING ANIMAL SECTIONS AND MOUNTING IN CANADA BALSAM. 



The preparations of borax carmine Logwood or haematoxylin 

 Staining processes Double staining with logwood and rosin 

 Mounting in Canada balsam - - 77-80 



LESSON 4. 



STAINING BLOOD AND EPITHELIUM, TEASING OUT TISSUES AND 

 MOUNTING IN AQUEOUS MEDIA. 



Double staining nucleated blood corpuscles Preparing blood of 

 mammals Non-nucleated corpuscles Epithelium Teasing 

 out muscle, fibrous tissue, yellow elastic tissue, nerve fibres, and 

 non-striped muscle Farrant's medium and glycerine jelly, 

 how prepared and used -' 80-83 



LESSON 5. 



STAINING AND MOUNTING MICRO-ORGANISMS. 



Staining and mounting micro-organisms Staining bacteria on 

 cover-glass Ehrlich's method of double -staining - 83-85 



