Injection of Bloodvessels. 85 



Oil of cloves is not suitable for clearing tissues stained 

 with anilin dyes ; it has a tendency to discharge the colour. 



LESSON 6. 

 INJECTION OF BLOODVESSELS. 



Carmine and Gelatine Injecting Mass. 



Pure carmine ... ,.. ... 60 grains. 



Liq. ammonia fort. ... ... 2 drams. 



Glacial acetic acid ... ... 86 minims. 



Gelatine solution (1 ounce in 6 ounces 



of water) ... ... ... 2 ounces. 



Water ... ... ... ... 2 ounces. 



Dissolve the carmine in the ammonia and water in a test- 

 tube, and mix it with one half of the warm gelatine. Add 

 the acid to the remaining half of gelatine, and drop it little 

 by little into the carmine mixture, stirring well all the time 

 with a stick or glass rod. Filter through flannel, and add a 

 few drops of carbolic acid to make the mass keep. The 

 principle to be attended to in making this mass is this : the 

 carmine, if alkaline, would diffuse through the vessels and 

 stain the tissues around them; if acid, the carmine would 

 be deposited in fine granules, which would block up the 

 capillaries ; hence the necessity for a neutral fluid. The 

 best guides are the colour and smell of the fluid. It should 

 be a bright red, and all trace of smell of ammonia must be 

 removed. The gelatine solution is made by putting 1 

 ounce of gelatine into 6 ounces of water ; it must then be 

 left until the gelatine becomes quite soft ; then dissolve over 

 a water- bath. 



Prussian or Berlin Blue and Gelatine Mass. Take 

 lj ounces of gelatine, place it in a vessel and cover 

 it with water ; allow it to stand until all the water is 

 absorbed and the gelatine is quite soft. Then dissolve in a 

 hot-water bath. Dissolve 1 dram of Prussian or Berlin 

 blue in 6 ounces of water, and gradually mix it with the 



6 



