Cutting, Staining, and Mounting Vegetable Sections. 87 



Injection of Lymphatics (Puncture Method). A small 

 subcutaneous syringe is filled with a watery solution of 

 Berlin or Prussian blue, and the nozzle is thrust into the 

 pad of a cat's foot. The injection is then to be forced into 

 the tissues. Then rub the limb from below upwards. This 

 will cause the injection- fluid to flow along the lymphatics, 

 and find its way into the glands of the groin. 



To Inject Lymph-sinuses of Glands. Force the nozzle 

 of a subcutaneous syringe into the hilum of a lymphatic 

 gland of an ox, and inject a watery solution of Prussian or 

 Berlin blue until the blue appears on the surface of the 

 gland. Then place it in methylated spirit to harden. 



When blue injection-fluid is used, add a few drops, of 

 acetic acid to the spirit while hardening the tissues. 



LESSON 7. 



CUTTING, STAINING, AND MOUNTING 

 VEGETABLE SECTIONS. 



Stems, leaves, roots, ovary, etc., should be hardened in 

 methylated spirit for a week or ten days, and the spirit 

 changed every twenty-four hours for the first three days. 

 The stems must not be too old. One, two, and three years' 

 growth will show all that is required. 



Section- cutting 1 , by hand and with a microtome, should 

 be done in the same manner as described on page 72. 



Bleaching. Vegetable sections generally require bleach- 

 ing before they can be properly stained. Chlorinated soda 

 is used for this purpose. Take of dry chloride of lime 

 2 ounces, of washing soda 4 ounces, and distilled water 

 2 pints. Mix the lime in one pint of the water and dissolve 

 the soda in the other. Mix the two solutions together, 

 shake well, and let the mixture stand for twenty-four hours. 

 Pour off the clear fluid, filter, and keep in a stoppered bottle 

 in a dark place, or cover the bottle with paper. Soak the 

 sections in distilled water. Pour off the water and add a 

 quantity of bleaching fluid. Allow this to act for from 



