88 Modern Microscopy. 



one to twelve hours. Wash well in water, which must be 

 changed several times to remove all trace of soda. The 

 sections may now be stained, or they may be preserved in 

 spirit until required. 



Staining Borax Carmine (suitable for ovaries, fruits, 

 etc.). Pure carmine 1 dram, liq. ammoniae fort. 2 drams. 

 Dissolve the carmine in the ammonia, and add 12 ounces 

 of a saturated solution of borax in distilled water. Filter 

 and keep in a stoppered bottle. 



(1) Put some stain in a watch-glass, and immerse the 

 section three to five minutes. 



(2) Wash well in methylated spirit. 



(3) Take of hydrochloric acid 1 part and of methylated 

 spirit 5 parts ; mix well together, and soak the section 

 until the colour changes to a bright scarlet, which takes 

 about five minutes. The acidulated spirit may be kept 

 ready for use at any time. 



(4) Wash well in methylated spirit. Then place in some 

 strong methylated spirit, and soak for at least ten minutes 

 to dehydrate. 



(5) Place the section on the surface of a small saucer of 

 clove oil, and let it soak until clear. 



(6) Kemove from the clove oil and place in turpentine, 

 and then mount in Canada balsam. 



Haematoxylin. Haematoxylin, 30 grains ; absolute 

 alcohol, 3J ounces ; distilled water, 3 J ounces ; glycerine, 

 3 ounces ; ammonia alum, 30 grains ; glacial acetic acid, 

 3 drams. Dissolve the haematoxylin in the alcohol and the 

 alum in the water ; then add the glycerine and acetic acid. 

 Mix the two solutions together, and let the mixture stand 

 for at least a month before use. 



(1) Add about 30 drops of the above to an ounce of dis- 

 tilled water, and stain the section for fifteen to thirty 

 minutes. 



(2) Wash well in distilled water, and then in ordinary 

 tap-water. This will fix the colour and make it deeper. 



