Catting, Staining, and Mounting Vegetable Tissues. 89 



(3) Dehydrate in strong methylated spirit for at least 

 ten minutes. 



(4) Clear in clove oil and mount in Canada balsam. 

 Double Staining. 



Green Stain. Acid green ... 2 grains. 



Distilled water ... 3 ounces. 

 Glycerine ... 1 ounce. 



Mix the water and glycerine together, and dissolve the 

 green in the mixture. 



Carmine Stain A. Borax ... ... 10 grains. 



Distilled water ... 1 ounce. 

 Glycerine . . J ounce. 



Alcohol rect. ... | ounce. 



Dissolve the borax in the water, and add the glycerine 

 and alcohol. 



B. Carmine ... ... 10 grains. 



Liq. ammoniae ... 20 min. 

 Distilled water ... 30 min. 



Dissolve the carmine in the water and ammonia. Mix 

 A and B together and filter. 



(1) Place the section in green stain for five to ten 

 minutes. 



(2) Wash in water. 



(3) Place in carmine for ten to fifteen minutes. 



(4) Wash well in methylated spirit. 



(5) Dehydrate and clear in clove oil. Wash in turpen- 

 tine and mount in Canada balsam. 



Mounting in Canada Balsam. Take 3 ounces of dried 

 Canada balsam and dissolve in 3 fluid ounces of benzole. 

 Filter and keep in an outside-stoppered bottle. 



(1) Clean a cover-glass, moisten the surface of a slide 

 with your breath, apply the cover-glass to it, and make 

 sure that it adheres. 



(2) Place a few drops of balsam on the cover-glass. 



(3) Take the section out of the turpentine on a lifter, 

 and put it into the balsam on the cover. 



