Cutting, Grinding, and Mounting Hard Tissues. 93 



metal. Vulcanite or glass cells must be used. To one side 

 of a cell apply a coat of shellac cement and let it dry ; now 

 take a slide and warm it over a spirit-lamp ; take up the 

 cell in a pair of forceps, and bring the cemented side in 

 contact with the centre of the warmed slide, and press it 

 down until it adheres firmly ; then add another coat of 

 cement to the upper side of the cell, and let it nearly dry, 

 put in the specimen, fill the cell with solution, and apply 

 the cover-glass. 



LESSON 9. 



CUTTING, GRINDING, AND MOUNTING 

 SECTIONS OF HARD TISSUES. 



Bone. Take the femur of a dog or cat, remove as much 

 of the muscles as possible, and macerate in water until 

 quite clean, then allow it to dry. 



(1) With a fine saw make transverse and longitudinal 

 sections. 



(2) Take a hone (water of Ayr stone), moisten it with 

 water, and rub one side of the section upon it until it is 

 quite flat and smooth. 



(3) Wash in water, and set aside until quite dry. 



(4) Take some, dried Canada balsam, place a piece on a 

 square glass, and warm gently over a lamp until the balsam 

 melts ; allow it to cool a little, and then press the smooth 

 side of the section into it, and set aside until cold. 



(5) With a fine file rub the section down as thin as 

 possible. 



(6) Take the hone again, and grind the section down 

 until thin enough, using plenty of water. 



(7) Place it, glass and all, in methylated spirit until the 

 section comes away from the glass, then wash well in clean 

 water. 



(8) Make a cell with any good shellac cement and allow 

 it to dry ; then apply another coat of cement, and after 

 waiting a few minutes fill the cell with camphor water, and 



