222 ' PARASITOLOGY. 



oil of cloves. The first clearing agent will perhaps 

 give the best results, or as soon as the worms are col- 

 lected and washed, dropped in an 80% alcohol at 80°. 

 This causes the worms to straighten out and kills them 

 at once. They are then placed in 80% alcohol to which 

 10% glycerin has been added then 20% glycerin grad- 

 ually increasing to full strength till the bodies are 

 clarified. The internal anatomy can now be studied. 

 The parasites may be stained with alcoholic eosin and 

 clarified in beechwood creosote and kept in small bot- 

 tles in creosote or mounted in Canada balsam. Care 

 must be exercised in placing them in the glycerine to 

 not let them stay too long lest they become too trans- 

 parent. 



PRESERVATION OF MUSEUM SPECIMENS. 



The Keiserling method gives the best results be- 

 cause the tissues retain their normal color. This 

 method consists of three steps as follows : 



1. Place in the following solution and leave from 

 one to seven days, depending upon the size of the 

 specimen : 



Formalin 200cc 



Potassium Acetate 30 grams 



Potassium Nitrate 15 grams 



Water 1000 cc 



2. Pass the specimen through each of the follow* 

 ing solutions, leaving it in each for twenty-four 

 hours. This should restore the normal color : 



40% Alcohol ■ 



60% Alcohol 



80% Alcohol 



95% Alcohol 



