224 PAIL\SITOLOGY. 



TO STAIN THE TRYPANOSOMA, 



1. Obtain blood smear as above. 



2. Air dry. 



3. Fix by dropping the slide in alcohol for two 

 minutes. 



4. Cover with methylene blue or gentian violet 

 one to three minutes. 



5. Wash in water. 



6. Air dry. 



7. Mount in Canada balsam. 



TO STAIN COCCIDIA, SARCOSPORIDIA OR 

 AMOEBA MELEAGRIDIS IN TISSUES. 



THE PREPARATION OF TISSUE. 



1. Secure block of tissue one-fourth inch square. 



2. Place in four percent formalin solution twenty- 

 four hours. 



3. Place in alcohol and ether equal parts twenty^ 

 four hours. 



4. Place in thin celloidin twenty-four hours. 



5. Place in thick celloidin twenty-four hours. 



6. Block on tile or wood. 



7. Cut eight to ten microns thick. 



TO STAIN THE PROTOZOA AND TISSUES. 



1. Cover section with Delafield's hematoxylin one- 

 half to two minutes. 



2. Wash in water. 



3. Stain two minutes in a one percent aqueous 

 eosin solution. 



