PARASITOLOGY. 225 



4. Wash 



5. Dehydrate with alcohol. 



6. Clarify with olive oil, xylol or cedar oil. 



7. Absorb clarifying fluid with blotting or filter 

 paper. 



8. Mount in Canada balsam. 



TO STAIN ENCYSTED TRICHINA. 



Prepare the block of tissue and section the same 

 as for coccidia. 



The following staining method stains the nuclei 

 of the cells violet or deep blue ; the cell bodies 

 and intercellular substance red : 



1. Immerse the section in hematoxylin three to 

 five minutes. 



2. Wash in water. 



3. Add a drop of weak hydrochloric acid 1 minutec 



4. Wash in water. 



5. i\queous eosin 2 minute:, 



6. Wash in water. 



7. Dehydrate in alcohol 3 minutes, 



8. Clarify in clove oil. 



9. Absorb clearing fluid. 

 10. Mount in Canada balsam. 



