24 



lene violet. Giemsa's mixture (procured ready made) 

 is then 



Azur II-Eosin 3 parts 



Azur II 0*8 part 



Glycerin - 250*0 parts 



Methyl alcohol - 250*0 



To Stain. (i) Fix in alcohol. (2) Mix one drop 

 of the solution with I c.c. of water. (3) Stain five to 

 ten minutes, or several hours. (4) Wash in water. 



Note. To obtain malignant stippling (p. 33) it is 

 recommended to add to 10 c.c. of solution one or two 

 drops of I per mille potassium carbonate solution. 



To obtain the most brilliant results with these 

 stains is perfectly easy, and no one who has used them 

 will, except for special reasons, use any others at 

 present in use. 



As stated above the simple Romanowsky stain is 

 as good as any of the modifications. 



To make fresh films. For studying movement, 

 delicacies of structure, for watching the process of 

 ex-flagellation, phagocytosis, fertilisation, and other 

 phenomena of the living parasite, it is necessary to be 

 able to make good fresh films. 



It is well to polish the slide and coverglass immedi- 

 ately before use with a clean handkerchief. When the 

 exuding drop of blood reaches the size of a small pin's 

 head, a coverglass is picked up rapidly with forceps, or 

 the edges are grasped between finger and thumb, and 

 allowed to touch the drop without ' dabbing ' the skin, 

 and then carefully dropped on to a slide. A gentle tap 

 or two with a needle or forceps may aid in the film 

 formation, but the pressure must not be great or the 

 corpuscles will be found laked and invisible. 



The requirements of a suitable wet film are. That 



