concentrated into a ring or figure of 8. Observe that 

 attached to the:e spherical bodies small ring-like 

 bodies occur, one to two in number, about as big as a 

 pin's head. These are the so-called ' polar ' bodies. 

 They occur in the male and female, and, as seen in 

 stained specimens, consist of little circular masses of 

 chromatin (?). These changes occur in the tropics 

 very rapidly, so that the examination must be com- 

 menced as rapidly as possible. 



On watching these spheres, the pigment in some 

 will be seen to be in active motion this probably 

 indicates the internal changes preparatory to extrusion 

 of flagella. Suddenly one of these spheres is per- 

 ceived to be oscillating violently, and in a moment 

 three or four or more pale, long processes are emitted 

 (Fig. 9). The red cells all around are put in motion 

 by their violence, and it may be only after a time, 

 when the activity has grown less, that the flagella are 

 actually seen. Nodosities will be observed in the 

 flagella, and occasionally a speck of pigment at their 

 extreme end. The flagella after a time break off, but 

 they have only once, by MacCallum, been seen 

 penetrating the female gamete. 



To STAIN FLAGELLATED BODIES 



When flagellation is observed the coverglass is 

 forcibly ' smeared ' off ; slide and coverglass are then 

 fixed, and stained with Romanowsky. 



Beautiful preparations are easily got by this 

 method (Fig. 9). 



THE SUBSIDIARY SIGNS OF MALARIA 



When patients have taken quinine it is not 

 uncommonly impossible to find parasites in the peri- 



