44 



Chapter IV 

 THE PARASITE IN THE TISSUES 



To Make Smear Preparations. Place a minute 

 portion of the tissue (e.g. brain, spleen) on a slide, and 

 with the end of another slide spread it out as evenly 

 and thinly as possible. Dry, fix, and stain in the same 

 way as a blood film. Parasites, if present, are in this 

 way much more easily and clearly seen than in sections. 

 Spleen pulp, bone marrow, kidney, liver, etc., give 

 beautiful results, and in the same way any secretion 

 or fluid can be examined. 



Fixation of Tissues. N.B. Use small pieces. 



1. Cut the tissues with a sharp knife or razor into 

 pieces not thicker than one-eighth of an inch (3 mm.) 



2. Place some cotton wool at the bottom of a 

 bottle or specimen tube. Pour in the fixative and 

 add the pieces of tissue. The amount of fixative to 

 be used should be about fifty times the volume of 

 the tissue. 



3. Pieces of intestine (e.g., in cases of malaria 

 cholerica) may be laid on pieces of paper and then put 

 in the fixative. The tissue adheres to the paper and 

 retains its shape. Proceed similarly for any thin tissue 

 or thin slice of an organ which it is required to embed 

 and get sections of rapidly. 



4. The results got with alcohol or formalin are 

 not nearly as good as with other fixatives which require 

 some trouble in the making. (Fide ch. xxxiv.) 



