4 8 



the slide is covered with a series of ribbons (if desired) 

 one beneath the other. Warm the sections very care- 

 fully over a small flame until the paraffin is stretched. 



4. Drain off the excess of water and then blot 

 off the remainder. Dry the sections in an ordinary 

 incubator 38*5 (about;; or by a fire, protected from 

 dust; or, for rough purposes, over a flame without 

 melting the paraffin wax. 



5. Allow xylol to trickle over the section till the 

 paraffin is dissolved out, or place in a pot of xylol. 



6. Wash off the xylol in alcohol and then bring 

 (through descending strengths of alcohol) into water. 

 (Fide ch. xxxiv.) 



STAINING 



Haematein. Stain five to twenty minutes accord- 

 ing to the activity of the stain as seen by the depth of 

 colour of the sections. If over-stained they may be 

 decolorized in one per cent, alum solution. Wash in 

 water. Counterstain with eosin (p. 408) for one 

 minute. 



Methylene Blue. Stain with one-fourth per cent, 

 methylene blue for one hour. Wash in water and 

 alcohol and counterstain with eosin. 



Romanowsky. It is difficult to get good results 

 with sections as indeed it is with thick blood films. 

 Formalin should not be used to fix. The sections 

 should be as thin as possible. Fulleborn recommends 

 the following method. 



(1) Giemsa stain, 10 drops, H 2 O 20 c.c. Stain 

 with two or three lots for several hours. 



(2) Wash off excess and then drop on acetic acid, 

 I in 1000, till violet. Wash in water. 



