49 



(3) Stain with the ordinary diluted Romanowsky 

 (for one hour). 



(4) Place in seventy per cent, alcohol and gradually 

 increase strength till absolute alcohol containing some 

 eosin is added. 



(5) Xylol. Balsam. 



To Mount Sections. Pass through successive 

 alcohols, oil of cloves (or xylol), to Canada balsam. 

 In hot moist climates, the cold produced by the 

 evaporation of the alcohol causes dew to be deposited 

 upon the slide. When the xylol or oil of cloves is 

 added, this produces a troublesome milkiness and may 

 spoil the section. To avoid this, all excess should be 

 rapidly wiped up after the use of alcohol, and the oil 

 of cloves added as quickly as possible. 



POST-MORTEM CHANGES IN MALARIA 



(MARCHIAFAVA AND BIGNAMI) 



Brain : 



1. Punctiform haemorrhages of the meninges. 



2. Punctiform haemorrhages of the white sub- 

 stance of the brain. 



3. The brain capillaries may have nearly every 

 red cell infected. Sporulating forms are especially 

 common. 



4. The capillary endothelium may show fatty 

 degeneration, together with pigmentation, and some- 

 times parasites. 



5. Similar appearances are also found in the 

 vessel of the pia mater. 



Lungs : 



I. Large pigmented mononuclears in the 

 capillaries, but especially in the veins ; in the lungs 

 especially, phagocytosis is proceeding. 



